Kremlin spokesman Dmitry Peskov told reporters Moscow was tired of the accusations.
He said a report released by US intelligence agencies detailing the allegations was groundless.
It is the first official reaction from Russia since President-elect Donald Trump received the report on Friday.
The unclassified report contains allegations that Russian President Vladimir Putin had ordered the hacking of Democratic Party emails to damage Donald Trump's Democrat rival, Hillary Clinton, and influence the election.
In his comments on Monday, Mr Peskov said Russia "categorically denied that Moscow had been involved in any hacking attacks".
"Groundless accusations which are not supported by anything are being rehearsed in an amateurish, unprofessional way. We don't know what information they are actually relying on."
The claims amounted to a "witch-hunt", he added.
Key findings of report (unclassified version):
- Kremlin had "clear preference" for Mr Trump to win US election, with goal to "undermine public faith in US democratic process and "denigrate" Hillary Clinton
- Russian military intelligence hacked into the email accounts of the Democratic National Committee and top Democrats
- They used intermediaries such as WikiLeaks, DCLeaks.com and Guccifer 2.0 persona to release the information acquired from the hackings
- They also used state-funded propaganda and paid social media users or "trolls" to make nasty comments
Vote tallies were not affected by Russian interference
Mr Trump used the same "witch-hunt" term last week in a New York Times interview to disparage the hacking claims, which he has repeatedly rejected since winning the presidential election in November.
But his incoming chief of staff, Reince Priebus, told Fox News Sunday that the president-elect had accepted the findings of the report, which was presented to him by intelligence chiefs on Friday.
"He's not denying that entities in Russia were behind this particular campaign," Mr Priebus added.
He did not clarify whether Mr Trump believed the report's assertion that Russian President Vladimir Putin had directly ordered the hack.
Mr Trump described his meeting on Friday with National Intelligence Director James Clapper, CIA Director John Brennan and FBI Director James Comey as "constructive" and said he would ask, within 90 days of taking office, for a plan on how to stop cyber attacks.
With less than two weeks until his inauguration, Mr Trump is under increasing pressure from both Democrats and Republicans to respond to the allegations.
President Obama has already expelled 35 Russian diplomats from US soil over the hacking. Russia said it would not reciprocate.